TY - JOUR
T1 - Functional dysregulation of dendritic cells in patients with papular urticaria caused by fleabite
AU - Cuéllar, Adriana
AU - García, Elizabeth
AU - Rodríguez, Adriana
AU - Halpert, Evelyne
AU - Gómez, Alberto
PY - 2007/11
Y1 - 2007/11
N2 - Background: Papular urticaria is a chronic allergic disease caused by fleabite. The presence of eosinophils, predominance of CD4-positive T cells in lesions, and IgE response suggest a Th2 immune response to flea proteins in patients with papular urticaria caused by fleabite (PUFB). Although PUFB is defined as an allergic reaction, the immunological mechanisms and the role of dendritic cells (DCs) have not been established. Observations: Flea body extract did not induce the maturation of monocyte-derived DCs in 10 patients with PUFB and in 10 healthy children. Simultaneous exposure of DCs to flea extract and lipopolysaccharide induced increased expression of CD83 (P <.01), CD86 (P <.01), and HLA-DR (P <.05), which was statistically significantly greater in patients' cells. Dendritic cells from patients stimulated with lipopolysaccharide secreted less interleukin 6 (IL-6) and IL-10 than DCs from control subjects. Conclusions: Results of this study indicate that the involvement of DCs in an immune response produced in the disease is mediated through the altered expression of membrane molecules. This may be related to constitutive impairment in the production of regulatory cytokines such as IL-6 and IL-10 in these patients.
AB - Background: Papular urticaria is a chronic allergic disease caused by fleabite. The presence of eosinophils, predominance of CD4-positive T cells in lesions, and IgE response suggest a Th2 immune response to flea proteins in patients with papular urticaria caused by fleabite (PUFB). Although PUFB is defined as an allergic reaction, the immunological mechanisms and the role of dendritic cells (DCs) have not been established. Observations: Flea body extract did not induce the maturation of monocyte-derived DCs in 10 patients with PUFB and in 10 healthy children. Simultaneous exposure of DCs to flea extract and lipopolysaccharide induced increased expression of CD83 (P <.01), CD86 (P <.01), and HLA-DR (P <.05), which was statistically significantly greater in patients' cells. Dendritic cells from patients stimulated with lipopolysaccharide secreted less interleukin 6 (IL-6) and IL-10 than DCs from control subjects. Conclusions: Results of this study indicate that the involvement of DCs in an immune response produced in the disease is mediated through the altered expression of membrane molecules. This may be related to constitutive impairment in the production of regulatory cytokines such as IL-6 and IL-10 in these patients.
UR - http://www.scopus.com/inward/record.url?scp=36348985003&partnerID=8YFLogxK
U2 - 10.1001/archderm.143.11.1415
DO - 10.1001/archderm.143.11.1415
M3 - Article
C2 - 18025366
AN - SCOPUS:36348985003
SN - 0003-987X
VL - 143
SP - 1415
EP - 1419
JO - Archives of Dermatology
JF - Archives of Dermatology
IS - 11
ER -