Frequency of specific CD8+ T cells for a promiscuous epitope derived from Trypanosoma cruzi KMP-11 protein in chagasic patients

P. Lasso, D. Mesa, A. Cuéllar, F. Guzmán, N. Bolaños, F. Rosas, V. Velasco, M. Del Carmen Thomas, M. C. Lopez, J. M. Gonzalez, C. J. Puerta

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The K1 peptide is a CD8+T cell HLA-A*0201-restricted epitope derived from the Trypanosoma cruzi KMP-11 protein. We have previously shown that this peptide induces IFN-γ secretion by CD8+T cells. The aim of this study was to characterize the frequency of K1-specific CD8 +T cells in chagasic patients. Nineteen HLA-A2+individuals were selected from 50 T. cruzi infected patients using flow cytometry and SSP-PCR assays. Twelve HLA-A*0201+noninfected donors were included as controls. Peripheral blood mononuclear cells were stained with HLA-A2-K1 tetramer, showing that 15 of 19 infected patients have K1-specific CD8+T cells (0·09-0·34% frequency) without differences in disease stages or severity. Of note, five of these responders were A*0205, A*0222, A*0226, A*0259 and A*0287 after molecular typing. Thus, a phenotypic and functional comparison of K1-specific CD8+T cells from non-HLA-A*0201 and HLA-A*0201 +infected patients was performed. The results showed that both non-HLA-A*0201 and HLA-A*0201+individuals have a predominant effector memory CD8+T cell phenotype (CCR7-, CD62L-). Moreover, CD8+T cells from non-HLA-A*0201 and HLA-A*0201+individuals expressed IL-2, IFN-γ and perforin without any differences. These findings support that K1 peptide is a promiscuous epitope presented by HLA-A2 supertype molecules and is highly recognized by chagasic patients.

Original languageEnglish
Pages (from-to)494-502
Number of pages9
JournalParasite Immunology
Volume32
Issue number7
DOIs
StatePublished - Jul 2010

Keywords

  • CD8T cell
  • Chagas' disease
  • HLA-A2 supertype
  • Trypanosoma cruzi

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