False positive serology of prepandemic chagasic samples with SARS-CoV-2 antigen

Juan D.H. Zuluaga; Juan C. Santos-Barbosa; Adriana Cuellar; Concepción J. Puerta; John M. Gonzalez

doi:10.1111/tmi.13818

False positive serology of prepandemic chagasic samples with SARS-CoV-2 antigen

Juan D.H. Zuluaga, Juan C. Santos-Barbosa, Adriana Cuellar, Concepción J. Puerta, John M. Gonzalez

Department of Microbiology

Universidad de los Andes Colombia

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Objective: To determine whether prepandemic sera from patients with Chagas disease recognise SARS-CoV-2 antigens. Materials and Methods: Forty sera from patients with Chagas disease were tested for the presence of IgG cross-reactivity against the nucleocapsid protein (NP) and spike (S) SARS-CoV-2 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP. Results: None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti-Trypanosoma cruzi antibodies reacted against the SARS-CoV-2 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re-tested, five remained positive by ELISA and all were negative by CLIA. Conclusion: According to our data, false-positive results might be a concern in the detection of SARS-CoV-2 antibodies in patients with Chagas disease.

Original language	English
Pages (from-to)	1009-1012
Number of pages	4
Journal	Tropical Medicine and International Health
Volume	27
Issue number	11
DOIs	https://doi.org/10.1111/tmi.13818
State	Published - Nov 2022

Keywords

COVID-19
Chagas disease
SARS-CoV-2
antibody cross-reactivity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/tmi.13818

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@article{6c8838d2aad54cdeb0de97560e981199,

title = "False positive serology of prepandemic chagasic samples with SARS-CoV-2 antigen",

abstract = "Objective: To determine whether prepandemic sera from patients with Chagas disease recognise SARS-CoV-2 antigens. Materials and Methods: Forty sera from patients with Chagas disease were tested for the presence of IgG cross-reactivity against the nucleocapsid protein (NP) and spike (S) SARS-CoV-2 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP. Results: None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti-Trypanosoma cruzi antibodies reacted against the SARS-CoV-2 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re-tested, five remained positive by ELISA and all were negative by CLIA. Conclusion: According to our data, false-positive results might be a concern in the detection of SARS-CoV-2 antibodies in patients with Chagas disease.",

keywords = "COVID-19, Chagas disease, SARS-CoV-2, antibody cross-reactivity",

author = "Zuluaga, {Juan D.H.} and Santos-Barbosa, {Juan C.} and Adriana Cuellar and Puerta, {Concepci{\'o}n J.} and Gonzalez, {John M.}",

note = "Publisher Copyright: {\textcopyright} 2022 John Wiley & Sons Ltd.",

year = "2022",

month = nov,

doi = "10.1111/tmi.13818",

language = "English",

volume = "27",

pages = "1009--1012",

journal = "Tropical Medicine and International Health",

issn = "1360-2276",

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T1 - False positive serology of prepandemic chagasic samples with SARS-CoV-2 antigen

AU - Zuluaga, Juan D.H.

AU - Santos-Barbosa, Juan C.

AU - Cuellar, Adriana

AU - Puerta, Concepción J.

AU - Gonzalez, John M.

PY - 2022/11

Y1 - 2022/11

N2 - Objective: To determine whether prepandemic sera from patients with Chagas disease recognise SARS-CoV-2 antigens. Materials and Methods: Forty sera from patients with Chagas disease were tested for the presence of IgG cross-reactivity against the nucleocapsid protein (NP) and spike (S) SARS-CoV-2 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP. Results: None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti-Trypanosoma cruzi antibodies reacted against the SARS-CoV-2 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re-tested, five remained positive by ELISA and all were negative by CLIA. Conclusion: According to our data, false-positive results might be a concern in the detection of SARS-CoV-2 antibodies in patients with Chagas disease.

AB - Objective: To determine whether prepandemic sera from patients with Chagas disease recognise SARS-CoV-2 antigens. Materials and Methods: Forty sera from patients with Chagas disease were tested for the presence of IgG cross-reactivity against the nucleocapsid protein (NP) and spike (S) SARS-CoV-2 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP. Results: None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti-Trypanosoma cruzi antibodies reacted against the SARS-CoV-2 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re-tested, five remained positive by ELISA and all were negative by CLIA. Conclusion: According to our data, false-positive results might be a concern in the detection of SARS-CoV-2 antibodies in patients with Chagas disease.

KW - COVID-19

KW - Chagas disease

KW - SARS-CoV-2

KW - antibody cross-reactivity

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SN - 1360-2276

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SP - 1009

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JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 11

ER -

False positive serology of prepandemic chagasic samples with SARS-CoV-2 antigen

Abstract

Keywords

UN SDGs

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