Evaluation of the integrity of the dopamine system in a rodent model of parkinson's disease: Small animal positron emission tomography compared to behavioral assessment and autoradiography

Elissa M. Strome; Ivan L. Cepeda; Vesna Sossi; Doris J. Doudet

doi:10.1007/s11307-006-0051-6

Evaluation of the integrity of the dopamine system in a rodent model of parkinson's disease: Small animal positron emission tomography compared to behavioral assessment and autoradiography

Elissa M. Strome
, Ivan L. Cepeda
, Vesna Sossi
, Doris J. Doudet

University of British Columbia

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Purpose: In the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD), it is important to determine lesion severity. This evaluation can be performed in vivo, through evaluation of dopamine (DA)-dependent motor function or with small animal positron emission tomography (microPET), or at postmortem, by examining markers for DA neurons. Procedures: Rats were given mild or severe unilateral 6-OHDA lesions, scanned with the tracer [¹¹C](±)dihydrotetrabenazine ([¹¹C]DTBZ), and tested on a tapered/ledged beam-walking task. At postmortem, autoradiography was performed with [¹¹C]DTBZ. Results: Autoradiography was significantly correlated with microPET and behavioral scores, whereas the microPET and behavioral data were not significantly correlated. Conclusions: This study shows that behavioral analysis, microPET, and autoradiography are all good tools for measuring the integrity of the DA system, and demonstrates the utility of the tapered/ledged beam-walking test to screen for lesion severity, as well as the importance of including postmortem analysis after in vivo imaging studies.

Original language	English
Pages (from-to)	292-299
Number of pages	8
Journal	Molecular Imaging and Biology
Volume	8
Issue number	5
DOIs	https://doi.org/10.1007/s11307-006-0051-6
State	Published - Sep 2006
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

6-OHDA
Behavior
Dopamine
MicroPET
Partial volume effect
Rodent
Striatum
Tapered beam-walking task
VMAT [C]DTBZ

Access to Document

10.1007/s11307-006-0051-6

Cite this

@article{1be65bac8d8f4d348c3c73bd1a36f516,

title = "Evaluation of the integrity of the dopamine system in a rodent model of parkinson's disease: Small animal positron emission tomography compared to behavioral assessment and autoradiography",

abstract = "Purpose: In the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD), it is important to determine lesion severity. This evaluation can be performed in vivo, through evaluation of dopamine (DA)-dependent motor function or with small animal positron emission tomography (microPET), or at postmortem, by examining markers for DA neurons. Procedures: Rats were given mild or severe unilateral 6-OHDA lesions, scanned with the tracer [11C](±)dihydrotetrabenazine ([11C]DTBZ), and tested on a tapered/ledged beam-walking task. At postmortem, autoradiography was performed with [11C]DTBZ. Results: Autoradiography was significantly correlated with microPET and behavioral scores, whereas the microPET and behavioral data were not significantly correlated. Conclusions: This study shows that behavioral analysis, microPET, and autoradiography are all good tools for measuring the integrity of the DA system, and demonstrates the utility of the tapered/ledged beam-walking test to screen for lesion severity, as well as the importance of including postmortem analysis after in vivo imaging studies.",

keywords = "6-OHDA, Behavior, Dopamine, MicroPET, Partial volume effect, Rodent, Striatum, Tapered beam-walking task, VMAT [C]DTBZ",

author = "Strome, \{Elissa M.\} and Cepeda, \{Ivan L.\} and Vesna Sossi and Doudet, \{Doris J.\}",

note = "Funding Information: Acknowledgments. We are grateful for technical assistance from Siobhan McCormick (microPET technician), Rick Kornelsen, and Jessica Grant (animal technicians), and to the staff at the UBC Animal Resource Unit. The authors would also like to acknowledge the staff of the UBC/TRIUMF PET group, and in particular Dr. Tom Ruth (Head), for their support of these studies. This work was supported by The Canadian Foundation for Innovation, The Canadian Institutes of Health Research, and the Michael Smith Foundation for Health Research. A version of this work was presented at the Academy of Molecular Imaging 2005 Annual Meeting, Orlando, FL.",

year = "2006",

month = sep,

doi = "10.1007/s11307-006-0051-6",

language = "English",

volume = "8",

pages = "292--299",

journal = "Molecular Imaging and Biology",

issn = "1536-1632",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "5",

}

Evaluation of the integrity of the dopamine system in a rodent model of parkinson's disease: Small animal positron emission tomography compared to behavioral assessment and autoradiography. / Strome, Elissa M.; Cepeda, Ivan L.; Sossi, Vesna et al.
In: Molecular Imaging and Biology, Vol. 8, No. 5, 09.2006, p. 292-299.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Evaluation of the integrity of the dopamine system in a rodent model of parkinson's disease

T2 - Small animal positron emission tomography compared to behavioral assessment and autoradiography

AU - Strome, Elissa M.

AU - Cepeda, Ivan L.

AU - Sossi, Vesna

AU - Doudet, Doris J.

N1 - Funding Information: Acknowledgments. We are grateful for technical assistance from Siobhan McCormick (microPET technician), Rick Kornelsen, and Jessica Grant (animal technicians), and to the staff at the UBC Animal Resource Unit. The authors would also like to acknowledge the staff of the UBC/TRIUMF PET group, and in particular Dr. Tom Ruth (Head), for their support of these studies. This work was supported by The Canadian Foundation for Innovation, The Canadian Institutes of Health Research, and the Michael Smith Foundation for Health Research. A version of this work was presented at the Academy of Molecular Imaging 2005 Annual Meeting, Orlando, FL.

PY - 2006/9

Y1 - 2006/9

N2 - Purpose: In the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD), it is important to determine lesion severity. This evaluation can be performed in vivo, through evaluation of dopamine (DA)-dependent motor function or with small animal positron emission tomography (microPET), or at postmortem, by examining markers for DA neurons. Procedures: Rats were given mild or severe unilateral 6-OHDA lesions, scanned with the tracer [11C](±)dihydrotetrabenazine ([11C]DTBZ), and tested on a tapered/ledged beam-walking task. At postmortem, autoradiography was performed with [11C]DTBZ. Results: Autoradiography was significantly correlated with microPET and behavioral scores, whereas the microPET and behavioral data were not significantly correlated. Conclusions: This study shows that behavioral analysis, microPET, and autoradiography are all good tools for measuring the integrity of the DA system, and demonstrates the utility of the tapered/ledged beam-walking test to screen for lesion severity, as well as the importance of including postmortem analysis after in vivo imaging studies.

AB - Purpose: In the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD), it is important to determine lesion severity. This evaluation can be performed in vivo, through evaluation of dopamine (DA)-dependent motor function or with small animal positron emission tomography (microPET), or at postmortem, by examining markers for DA neurons. Procedures: Rats were given mild or severe unilateral 6-OHDA lesions, scanned with the tracer [11C](±)dihydrotetrabenazine ([11C]DTBZ), and tested on a tapered/ledged beam-walking task. At postmortem, autoradiography was performed with [11C]DTBZ. Results: Autoradiography was significantly correlated with microPET and behavioral scores, whereas the microPET and behavioral data were not significantly correlated. Conclusions: This study shows that behavioral analysis, microPET, and autoradiography are all good tools for measuring the integrity of the DA system, and demonstrates the utility of the tapered/ledged beam-walking test to screen for lesion severity, as well as the importance of including postmortem analysis after in vivo imaging studies.

KW - 6-OHDA

KW - Behavior

KW - Dopamine

KW - MicroPET

KW - Partial volume effect

KW - Rodent

KW - Striatum

KW - Tapered beam-walking task

KW - VMAT [C]DTBZ

UR - https://www.scopus.com/pages/publications/33749024759

U2 - 10.1007/s11307-006-0051-6

DO - 10.1007/s11307-006-0051-6

M3 - Article

C2 - 16897319

AN - SCOPUS:33749024759

SN - 1536-1632

VL - 8

SP - 292

EP - 299

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 5

ER -

Evaluation of the integrity of the dopamine system in a rodent model of parkinson's disease: Small animal positron emission tomography compared to behavioral assessment and autoradiography

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this