Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

F. Gamboa; J. M. Manterola; B. Vinado; L. Matas; M. Gimenez; J. Lonca; J. R. Manzano; C. Rodrigo; P. J. Cardona; E. Padilla; J. Dominguez; V. Ausina

doi:10.1128/jcm.35.1.307-310.1997

Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

F. Gamboa, J. M. Manterola, B. Vinado, L. Matas, M. Gimenez, J. Lonca, J. R. Manzano, C. Rodrigo, P. J. Cardona, E. Padilla, J. Dominguez, V. Ausina

Generalitat de Catalunya

Research output: Contribution to journal › Article › peer-review

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Abstract

The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.

Original language	English
Pages (from-to)	307-310
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	35
Issue number	1
DOIs	https://doi.org/10.1128/jcm.35.1.307-310.1997
State	Published - 1997
Externally published	Yes

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10.1128/jcm.35.1.307-310.1997

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Gamboa, F., Manterola, J. M., Vinado, B., Matas, L., Gimenez, M., Lonca, J., Manzano, J. R., Rodrigo, C., Cardona, P. J., Padilla, E., Dominguez, J., & Ausina, V. (1997). Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test. Journal of Clinical Microbiology, 35(1), 307-310. https://doi.org/10.1128/jcm.35.1.307-310.1997

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title = "Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test",

abstract = "The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.",

author = "F. Gamboa and Manterola, {J. M.} and B. Vinado and L. Matas and M. Gimenez and J. Lonca and Manzano, {J. R.} and C. Rodrigo and Cardona, {P. J.} and E. Padilla and J. Dominguez and V. Ausina",

year = "1997",

doi = "10.1128/jcm.35.1.307-310.1997",

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Gamboa, F, Manterola, JM, Vinado, B, Matas, L, Gimenez, M, Lonca, J, Manzano, JR, Rodrigo, C, Cardona, PJ, Padilla, E, Dominguez, J & Ausina, V 1997, 'Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test', Journal of Clinical Microbiology, vol. 35, no. 1, pp. 307-310. https://doi.org/10.1128/jcm.35.1.307-310.1997

TY - JOUR

T1 - Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

AU - Gamboa, F.

AU - Manterola, J. M.

AU - Vinado, B.

AU - Matas, L.

AU - Gimenez, M.

AU - Lonca, J.

AU - Manzano, J. R.

AU - Rodrigo, C.

AU - Cardona, P. J.

AU - Padilla, E.

AU - Dominguez, J.

AU - Ausina, V.

PY - 1997

Y1 - 1997

N2 - The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.

AB - The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.

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U2 - 10.1128/jcm.35.1.307-310.1997

DO - 10.1128/jcm.35.1.307-310.1997

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C2 - 8968935

AN - SCOPUS:0031060049

SN - 0095-1137

VL - 35

SP - 307

EP - 310

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 1

ER -

Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

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