Diminished mitogen-induced T cell proliferation by Trypanosoma cruzi antigens associated with antigen-presenting cell modulation and CD3 signaling

Sergio Gómez-Olarte, Natalia I. Bolaños, Adriana Cuéllar, Concepción J. Puerta, John M. González

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Chronic infection by Trypanosoma cruzi decreases T cell proliferation and it is most likely accompanied by changes in signals required for activation. We assessed the effect of T. cruzi antigens on mitogen-induced proliferation of T cells from uninfected individuals and the association with the expression of molecules involved in antigen presentation, T cell costimulation and activation, and cytokine production. T. cruzi antigen exposure reduced mitogen-induced proliferation of CD4+ and CD8+ T cells in PBMC cultures, but only reduced mitogen-induced proliferation in the CD4+ T cells from sorted cell cultures cocultured with antigen-pulsed CD3− cells. CD40/CD80 and CD86 expression were reduced in antigen-pulsed DCs and monocytes, respectively. TNF-α, IL-10 and CCL17 levels were increased in cultures with antigen-pulsed CD3− cells, while CD3ζ chain expression was reduced in T cells from cultures with antigen. Our findings suggest that T. cruzi could alter T cell proliferation indirectly by downregulating costimulatory molecules and inducing the secretion of IL-10 and directly by decreasing TCR signaling.

Original languageEnglish
Article number103974
JournalCellular Immunology
Volume348
DOIs
StatePublished - Feb 2020

Keywords

  • Antigen-presenting cells
  • Cell proliferation
  • Costimulatory molecules
  • Cytokines
  • T-lymphocyte subsets
  • TCR-CD3 complex
  • Trypanosoma cruzi

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