TY - JOUR
T1 - Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples
AU - Castellar-Mendoza, Camilo
AU - Calderón-Peláez, María Angélica
AU - Castellanos, Jaime E.
AU - Velandia-Romero, Myriam L.
AU - Coronel-Ruiz, Carolina
AU - Camacho-Ortega, Sigrid
AU - Bernal-Cepeda, Lilia J.
AU - López-Ibarra, Lady
AU - Arturo, Jhann A.
AU - Delgado, Félix G.
AU - Gutierrez-Barbosa, Hernando
AU - Bohorquez-Avila, Sonia
AU - Madroñero, Johanna
AU - Corredor-Rozo, Zayda L.
AU - Perdomo-Lara, Sandra J.
AU - Fonseca-Benitez, Angela
AU - Calvo, Eliana
N1 - Publisher Copyright:
© 2024 Camilo Castellar-Mendoza et al.
PY - 2024/3/11
Y1 - 2024/3/11
N2 - PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately.
AB - PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately.
UR - https://www.scopus.com/pages/publications/85188661551
U2 - 10.1155/2024/4894004
DO - 10.1155/2024/4894004
M3 - Article
AN - SCOPUS:85188661551
SN - 1687-918X
VL - 2024
JO - International Journal of Microbiology
JF - International Journal of Microbiology
M1 - 4894004
ER -