Determination of gene flow events in natural "wild-weedy-cultivated" complex in genepools of Phaseolus lunatus L

Gene flow events in natural populations of Lima beans have been reported along its broad range ofdistribution in the Americas (Mexico to Argentina) (Baudoin et al. 2004), as a result of its facultativereproduction system that allows varying levels of allogamy up to 48% (Maquet et al. 1996, and Zoro Bi et al.2005). We were interested in testing evidence of gene flow events using weedy materials from six populationsof Campeche (Mexico) (Debouck 1979) and ten populations from Cajamarca (Peru) (Debouck et al. 1987).The natural populations were chosen and analyzed using the same methodology as described by González-Torres et al. (2003, 2004) using morphoagronomic markers (Table). We evaluated the participation of nucleargenome through 18 microsatellite loci (Gaitan-Solís et al. 2002), and lectin patterns (Gutiérrez Salgado et al.1995). In order to establish the direction of the gene transfer we studied 25 non-coding regions of chloroplastDNA using RFLPs (Fofana et al. 1999, Chacón 2001, and González-Torres et al. 2003). On the other hand,the concentration of HCN has been evaluated (Essers et al. 1993) as an antinutritional compound in order toassess some consequences of gene flow in these populations.