Abstract
INTRODUCTION: Human umbilical cord blood (HUCB) is a common source of hematopoietic progenitor cells; however, coexpression of CD34, CD38 and HLA DR antigens and of embryonic antigens (SSEA-4 and CD13) have not been described in HUCB. OBJECTIVE: The current study attempted to identify in HUCB the presence of these antigens. MATERIALS AND METHODS: Cord blood samples were obtained from 80 women with normal pregnancies, who were attending the gynecology-obstetrics service of the San Ignacio Hospital, Bogotá (Colombia). The antigens were identified with a FACS Calibur BDO flow cytometer and a cocktail of monoclonal antibodies. RESULTS: The population of CD34+ cells in HUCB was higher than 0.2% (p = 0.001). The levels of additional cell subpopulations were as follows: CD34+/CD38-/HLA-DR--0.015%-0.023%, CD34+/CD38+/HLA-DR--0.019%-0.30%, CD34+/CD38-/HLA-DR(+)--0.043%-0.068% and CD34+/CD38+/HLA-DR--0.24%-0.51%. Eight samples of the eighty were positive for embryonic markers. The most frequent phenotype in HUCB was CD34+ CD38+ HLA DR+. CONCLUSION: This is the first time that SSEA-4 and CD13 antigens have been reported in human umbilical cord blood cells.
Translated title of the contribution | Phenotypical determinants of stem cell subpopulations derived from human umbilical cord blood |
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Original language | Spanish |
Pages (from-to) | 51-60 |
Number of pages | 10 |
Journal | Biomedica |
Volume | 26 |
Issue number | 1 |
DOIs | |
State | Published - Mar 2006 |