Characterization of rotavirus specific B cells and their relation with serological memory

Olga Lucía Rojas; Carlos Fernando Narváez; Harry B. Greenberg; Juana Angel; Manuel A. Franco

doi:10.1016/j.virol.2008.08.004

Characterization of rotavirus specific B cells and their relation with serological memory

Olga Lucía Rojas
, Carlos Fernando Narváez
, Harry B. Greenberg
, Juana Angel
, Manuel A. Franco

Institute of Human Genetics

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We quantified circulating total, rotavirus (RV) and Tetanus toxin (TT) memory B cells (mBc) in healthy adults using a limiting dilution assay (LDA) and a flow cytometry assay (FCA) that permit evaluation of both CD27+ and CD27- mBc. RV mBc were enriched in the CD27-, IgG+ and in the CD27+, IgM+ subsets. The numbers of RV mBc were higher by FCA than by LDA and results of the two assays did not correlate. TT IgGmBc and RV IgA mBc determined by FCA and by LDA correlated with TT plasma IgG and RV plasma IgA, respectively. The mean ratio of specific mBc/μg/ml of the corresponding plasma immunoglobulin was lower for TT IgG than for RV IgA mBc. Our studies contribute to understand the relationship between circulating mBc and serological memory, and enhance our capacity to develop better correlates of protection against RV disease.

Original language	English
Pages (from-to)	234-242
Number of pages	9
Journal	Virology
Volume	380
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.08.004
State	Published - 25 Oct 2008

Keywords

Flow cytometry assay
Limiting dilution assay
Memory B cell
Rotavirus

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10.1016/j.virol.2008.08.004

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title = "Characterization of rotavirus specific B cells and their relation with serological memory",

abstract = "We quantified circulating total, rotavirus (RV) and Tetanus toxin (TT) memory B cells (mBc) in healthy adults using a limiting dilution assay (LDA) and a flow cytometry assay (FCA) that permit evaluation of both CD27+ and CD27- mBc. RV mBc were enriched in the CD27-, IgG+ and in the CD27+, IgM+ subsets. The numbers of RV mBc were higher by FCA than by LDA and results of the two assays did not correlate. TT IgGmBc and RV IgA mBc determined by FCA and by LDA correlated with TT plasma IgG and RV plasma IgA, respectively. The mean ratio of specific mBc/μg/ml of the corresponding plasma immunoglobulin was lower for TT IgG than for RV IgA mBc. Our studies contribute to understand the relationship between circulating mBc and serological memory, and enhance our capacity to develop better correlates of protection against RV disease.",

keywords = "Flow cytometry assay, Limiting dilution assay, Memory B cell, Rotavirus",

author = "Rojas, \{Olga Luc{\'i}a\} and Narv{\'a}ez, \{Carlos Fernando\} and Greenberg, \{Harry B.\} and Juana Angel and Franco, \{Manuel A.\}",

note = "Funding Information: This work has been funded in part by the Pontificia Universidad Javeriana and in part by the FIRCA and NIH grants R03-TWO5647-04 and RO1-AI21362 and a VA Merit Review grant. The results were independently derived and do not reflect any endorsement on the part of the federal government or Stanford. We would like to thank N. Bernasconi, F. Sallusto and A. Lanzavecchia for help in setting up the LDA. C.F. Narv{\'a}ez is funded by Colciencias.",

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AU - Rojas, Olga Lucía

AU - Narváez, Carlos Fernando

AU - Greenberg, Harry B.

AU - Angel, Juana

AU - Franco, Manuel A.

N1 - Funding Information: This work has been funded in part by the Pontificia Universidad Javeriana and in part by the FIRCA and NIH grants R03-TWO5647-04 and RO1-AI21362 and a VA Merit Review grant. The results were independently derived and do not reflect any endorsement on the part of the federal government or Stanford. We would like to thank N. Bernasconi, F. Sallusto and A. Lanzavecchia for help in setting up the LDA. C.F. Narváez is funded by Colciencias.

PY - 2008/10/25

Y1 - 2008/10/25

N2 - We quantified circulating total, rotavirus (RV) and Tetanus toxin (TT) memory B cells (mBc) in healthy adults using a limiting dilution assay (LDA) and a flow cytometry assay (FCA) that permit evaluation of both CD27+ and CD27- mBc. RV mBc were enriched in the CD27-, IgG+ and in the CD27+, IgM+ subsets. The numbers of RV mBc were higher by FCA than by LDA and results of the two assays did not correlate. TT IgGmBc and RV IgA mBc determined by FCA and by LDA correlated with TT plasma IgG and RV plasma IgA, respectively. The mean ratio of specific mBc/μg/ml of the corresponding plasma immunoglobulin was lower for TT IgG than for RV IgA mBc. Our studies contribute to understand the relationship between circulating mBc and serological memory, and enhance our capacity to develop better correlates of protection against RV disease.

AB - We quantified circulating total, rotavirus (RV) and Tetanus toxin (TT) memory B cells (mBc) in healthy adults using a limiting dilution assay (LDA) and a flow cytometry assay (FCA) that permit evaluation of both CD27+ and CD27- mBc. RV mBc were enriched in the CD27-, IgG+ and in the CD27+, IgM+ subsets. The numbers of RV mBc were higher by FCA than by LDA and results of the two assays did not correlate. TT IgGmBc and RV IgA mBc determined by FCA and by LDA correlated with TT plasma IgG and RV plasma IgA, respectively. The mean ratio of specific mBc/μg/ml of the corresponding plasma immunoglobulin was lower for TT IgG than for RV IgA mBc. Our studies contribute to understand the relationship between circulating mBc and serological memory, and enhance our capacity to develop better correlates of protection against RV disease.

KW - Flow cytometry assay

KW - Limiting dilution assay

KW - Memory B cell

KW - Rotavirus

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Characterization of rotavirus specific B cells and their relation with serological memory

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Keywords

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