TY - JOUR
T1 - Chagasic patients are able to respond against a viral antigen from influenza virus
AU - Lasso, Paola
AU - Mesa, Diana
AU - Bolaños, Natalia
AU - Cuéllar, Adriana
AU - Guzmán, Fanny
AU - Cucunuba, Zulma
AU - Rosas, Fernando
AU - Velasco, Víctor
AU - Thomas, Maria C.
AU - López, Manuel C.
AU - González, John M.
AU - Puerta, Concepción J.
N1 - Funding Information:
The HLA-A2/Flu-MP* tetramers were generated and kindly provided by the NIH Tetramer Facility. This work was supported by Colciencias Research project No. 1203-333-18692, and COLCIENCIAS and CONICYT COLCIENCIAS and CONICYT 2008-151, Research Exchange Program. JMG was supported by an associate professor grant form Vicerrectoria Académica, Universidad de los Andes, Colombia. MC López and MC Thomas were supported by Grants P08-CVI-04037 from PAI (Junta de Andalucía), BFU2010-1670 from Plan Nacional I + D + i (MICINN) and RD06/0021/0014 from ISCIII-RETIC (MICINN Spain and FEDER).
PY - 2012/8/24
Y1 - 2012/8/24
N2 - Background: Trypanosoma cruzi, the etiological agent of Chagas' disease, is an obligate intracellular parasite which induces a CD8+ T cell immune response with secretion of cytokines and release of cytotoxic granules. Although an immune-suppressive effect of T. cruzi on the acute phase of the disease has been described, little is known about the capacity of CD8+ T cell from chronic chagasic patients to respond to a non-T. cruzi microbial antigen.Methods: In the present paper, the frequency, phenotype and the functional activity of the CD8+ T cells specific from Flu-MP*, an influenza virus epitope, were determined in 13 chagasic patients and 5 healthy donors.Results: The results show that Flu-MP* peptide specific CD8+ T cells were found with similar frequencies in both groups. In addition, Flu-MP* specific CD8+ T cells were distributed in the early or intermediate/late differentiation stages without showing enrichment of a specific sub-population. The mentioned Flu-MP* specific CD8+ T cells from chagasic patients were predominately TEM (CCR7- CD62L-), producing IL-2, IFNγ, CD107a/b and perforin, and did not present significant differences when compared with those from healthy donors.Conclusions: Our results support the hypothesis that there is no CD8+ T cell nonspecific immune-suppression during chronic Chagas disease infection. Nonetheless, other viral antigens must be studied in order to confirm our findings.
AB - Background: Trypanosoma cruzi, the etiological agent of Chagas' disease, is an obligate intracellular parasite which induces a CD8+ T cell immune response with secretion of cytokines and release of cytotoxic granules. Although an immune-suppressive effect of T. cruzi on the acute phase of the disease has been described, little is known about the capacity of CD8+ T cell from chronic chagasic patients to respond to a non-T. cruzi microbial antigen.Methods: In the present paper, the frequency, phenotype and the functional activity of the CD8+ T cells specific from Flu-MP*, an influenza virus epitope, were determined in 13 chagasic patients and 5 healthy donors.Results: The results show that Flu-MP* peptide specific CD8+ T cells were found with similar frequencies in both groups. In addition, Flu-MP* specific CD8+ T cells were distributed in the early or intermediate/late differentiation stages without showing enrichment of a specific sub-population. The mentioned Flu-MP* specific CD8+ T cells from chagasic patients were predominately TEM (CCR7- CD62L-), producing IL-2, IFNγ, CD107a/b and perforin, and did not present significant differences when compared with those from healthy donors.Conclusions: Our results support the hypothesis that there is no CD8+ T cell nonspecific immune-suppression during chronic Chagas disease infection. Nonetheless, other viral antigens must be studied in order to confirm our findings.
KW - CD8+ T cells
KW - Chagas disease
KW - Non-T. cruzi microbial antigen
KW - Nonspecific immune-suppression
UR - http://www.scopus.com/inward/record.url?scp=84865304499&partnerID=8YFLogxK
U2 - 10.1186/1471-2334-12-198
DO - 10.1186/1471-2334-12-198
M3 - Article
C2 - 22920436
AN - SCOPUS:84865304499
SN - 1471-2334
VL - 12
JO - BMC Infectious Diseases
JF - BMC Infectious Diseases
M1 - 198
ER -