TY - JOUR
T1 - Calcitonin Gene-related Peptide Receptor Expression in Alternatively Activated Monocytes/Macrophages During Irreversible Pulpitis
AU - Caviedes-Bucheli, Javier
AU - Moreno, Gloria Cristina
AU - López, María Paula
AU - Bermeo-Noguera, Ana Milena
AU - Pacheco-Rodríguez, Gloriana
AU - Cuellar, Adriana
AU - Muñoz, Hugo Roberto
PY - 2008/8
Y1 - 2008/8
N2 - The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental pulp. Pulp tissue samples were collected from teeth with a clinical diagnosis of irreversible pulpitis (n = 13), pulps with induced inflammation (n = 13), and normal pulps (n = 13). All samples were labeled to identify positive cells for CGRPr and CD163 using a flow cytometry assay. Results demonstrated that a high percentage of total viable AAMø CD163+ expressed CGRPr on their membranes (72.12% in healthy pulp, 62.20% in irreversible pulpitis, and 58.01% in induced pulpitis). Significant differences were found between mean AAMø CD163+ fluorescence for CGRPr according to pulp condition, being greater in irreversible pulpitis. It can be concluded that AAMø CD163+ are expressed during normal and inflammatory processes, supporting the hypothesis that they could exercise an anti-inflammatory action that could be controlled by CGRP signaling after its binding.
AB - The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental pulp. Pulp tissue samples were collected from teeth with a clinical diagnosis of irreversible pulpitis (n = 13), pulps with induced inflammation (n = 13), and normal pulps (n = 13). All samples were labeled to identify positive cells for CGRPr and CD163 using a flow cytometry assay. Results demonstrated that a high percentage of total viable AAMø CD163+ expressed CGRPr on their membranes (72.12% in healthy pulp, 62.20% in irreversible pulpitis, and 58.01% in induced pulpitis). Significant differences were found between mean AAMø CD163+ fluorescence for CGRPr according to pulp condition, being greater in irreversible pulpitis. It can be concluded that AAMø CD163+ are expressed during normal and inflammatory processes, supporting the hypothesis that they could exercise an anti-inflammatory action that could be controlled by CGRP signaling after its binding.
KW - Alternatively activated monocytes/macrophages
KW - CD163
KW - CGRP-receptor
KW - calcitonin gene-related peptide
KW - irreversible pulpitis
KW - neuropeptides
UR - http://www.scopus.com/inward/record.url?scp=47049113429&partnerID=8YFLogxK
U2 - 10.1016/j.joen.2008.05.011
DO - 10.1016/j.joen.2008.05.011
M3 - Article
C2 - 18634925
AN - SCOPUS:47049113429
SN - 0099-2399
VL - 34
SP - 945
EP - 949
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 8
ER -