TY - JOUR
T1 - Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale
AU - Blanco-Vargas, Andrea
AU - Rodríguez-Gacha, Lina M.
AU - Sánchez-Castro, Natalia
AU - Herrera-Carlosama, Laura
AU - Poutou-Piñales, Raúl A.
AU - Díaz-Ariza, Lucía A.
AU - Gutiérrez-Romero, Viviana
AU - Rivera-Hoyos, Claudia M.
AU - Ardila-Leal, Leidy D.
AU - Pedroza-Rodríguez, Aura M.
N1 - Publisher Copyright:
© Universitas Scientiarum, Journal of the Faculty of Sciences, Pontificia Universidad Javeriana, is licensed under the Creative Commons Attribution 4.0 International Public License
PY - 2021
Y1 - 2021
N2 - Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 gL-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse-and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5gL-1) fourfold lower than those in SMRS1 (10.0 gL-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08) log10 CFU mL-1 and, at 6h of culture, acid and alkaline phosphatase enzyme volumetric activities of (2.3 ± 0.8) UP and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17)mgL-1 at 6h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1 of bioinoculant plus 100kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables ((170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus) (p < 0.05).
AB - Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 gL-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse-and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5gL-1) fourfold lower than those in SMRS1 (10.0 gL-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08) log10 CFU mL-1 and, at 6h of culture, acid and alkaline phosphatase enzyme volumetric activities of (2.3 ± 0.8) UP and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17)mgL-1 at 6h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1 of bioinoculant plus 100kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables ((170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus) (p < 0.05).
KW - Allium cepa L.
KW - Bioinoculant
KW - Kosakonia sp
KW - Pseudomonas sp
KW - Serratia sp
KW - organic acids
KW - phosphatases
KW - phosphatases
KW - phosphate rock
UR - http://www.scopus.com/inward/record.url?scp=85104696799&partnerID=8YFLogxK
U2 - 10.11144/Javeriana.SC26-1.eobp
DO - 10.11144/Javeriana.SC26-1.eobp
M3 - Article
AN - SCOPUS:85104696799
SN - 0122-7483
VL - 26
SP - 79
EP - 118
JO - Universitas Scientiarum
JF - Universitas Scientiarum
IS - 1
ER -