Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

Andrea Blanco-Vargas, Lina M. Rodríguez-Gacha, Natalia Sánchez-Castro, Laura Herrera-Carlosama, Raúl A. Poutou-Piñales, Lucía A. Díaz-Ariza, Viviana Gutiérrez-Romero, Claudia M. Rivera-Hoyos, Leidy D. Ardila-Leal, Aura M. Pedroza-Rodríguez

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6 Scopus citations

Abstract

Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 gL-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse-and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5gL-1) fourfold lower than those in SMRS1 (10.0 gL-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08) log10 CFU mL-1 and, at 6h of culture, acid and alkaline phosphatase enzyme volumetric activities of (2.3 ± 0.8) UP and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17)mgL-1 at 6h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1 of bioinoculant plus 100kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables ((170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus) (p < 0.05).

Original languageEnglish
Pages (from-to)79-118
Number of pages40
JournalUniversitas Scientiarum
Volume26
Issue number1
DOIs
StatePublished - 2021

Keywords

  • Allium cepa L.
  • Bioinoculant
  • Kosakonia sp
  • Pseudomonas sp
  • Serratia sp
  • organic acids
  • phosphatases
  • phosphatases
  • phosphate rock

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