Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa

D. C. Becerra; A. P. Forero; G. A. Góngora

doi:10.1023/B:TICU.0000049440.10767.29

Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa

D. C. Becerra, A. P. Forero, G. A. Góngora

Department of Biology

Universidad Javeriana

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

A protocol for in vitro shoot regeneration from leaf discs of Passiflora edulis f. flavicarpa is described. The effect of explant source on morphogenesis was studied by comparing the response of explants collected from young plants, in vitro plantlets, and reinvigorated adult plants. Highest de novo shoot production was observed in explants from 2-month-old plants cultured in Murashige and Skoog medium supplemented with sucrose 30 g l ^-1, 4.44 μM benzyladenine, and 2.32 μM kinetin. Regenerated shoots rooted spontaneously in growth regulator-free medium and were successfully acclimatized to greenhouse conditions. Influence of the age and physiological state on shoot regeneration and gradual loss of morphogenic capacity is discussed.

Original language	English
Pages (from-to)	87-90
Number of pages	4
Journal	Plant Cell, Tissue and Organ Culture
Volume	79
Issue number	1
DOIs	https://doi.org/10.1023/B:TICU.0000049440.10767.29
State	Published - Oct 2004

Keywords

in vitro plant regeneration
maracuya
organogenesis
yellow passion fruit

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10.1023/B:TICU.0000049440.10767.29

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title = "Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa",

abstract = "A protocol for in vitro shoot regeneration from leaf discs of Passiflora edulis f. flavicarpa is described. The effect of explant source on morphogenesis was studied by comparing the response of explants collected from young plants, in vitro plantlets, and reinvigorated adult plants. Highest de novo shoot production was observed in explants from 2-month-old plants cultured in Murashige and Skoog medium supplemented with sucrose 30 g l -1, 4.44 μM benzyladenine, and 2.32 μM kinetin. Regenerated shoots rooted spontaneously in growth regulator-free medium and were successfully acclimatized to greenhouse conditions. Influence of the age and physiological state on shoot regeneration and gradual loss of morphogenic capacity is discussed.",

keywords = "in vitro plant regeneration, maracuya, organogenesis, yellow passion fruit",

author = "Becerra, {D. C.} and Forero, {A. P.} and G{\'o}ngora, {G. A.}",

note = "Funding Information: This work was supported by COLCIENCIAS, project 1203-12-097-98. The authors are grateful to Dr Ricardo Mart{\'i}nez for his collaboration with the statistical analysis, Claudia Ram{\'i}rez for helpful comments and Dr G.J. de Klerk for suggestions on the manuscript.",

year = "2004",

month = oct,

doi = "10.1023/B:TICU.0000049440.10767.29",

language = "English",

volume = "79",

pages = "87--90",

journal = "Plant Cell, Tissue and Organ Culture",

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T1 - Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa

AU - Becerra, D. C.

AU - Forero, A. P.

AU - Góngora, G. A.

N1 - Funding Information: This work was supported by COLCIENCIAS, project 1203-12-097-98. The authors are grateful to Dr Ricardo Martínez for his collaboration with the statistical analysis, Claudia Ramírez for helpful comments and Dr G.J. de Klerk for suggestions on the manuscript.

PY - 2004/10

Y1 - 2004/10

N2 - A protocol for in vitro shoot regeneration from leaf discs of Passiflora edulis f. flavicarpa is described. The effect of explant source on morphogenesis was studied by comparing the response of explants collected from young plants, in vitro plantlets, and reinvigorated adult plants. Highest de novo shoot production was observed in explants from 2-month-old plants cultured in Murashige and Skoog medium supplemented with sucrose 30 g l -1, 4.44 μM benzyladenine, and 2.32 μM kinetin. Regenerated shoots rooted spontaneously in growth regulator-free medium and were successfully acclimatized to greenhouse conditions. Influence of the age and physiological state on shoot regeneration and gradual loss of morphogenic capacity is discussed.

AB - A protocol for in vitro shoot regeneration from leaf discs of Passiflora edulis f. flavicarpa is described. The effect of explant source on morphogenesis was studied by comparing the response of explants collected from young plants, in vitro plantlets, and reinvigorated adult plants. Highest de novo shoot production was observed in explants from 2-month-old plants cultured in Murashige and Skoog medium supplemented with sucrose 30 g l -1, 4.44 μM benzyladenine, and 2.32 μM kinetin. Regenerated shoots rooted spontaneously in growth regulator-free medium and were successfully acclimatized to greenhouse conditions. Influence of the age and physiological state on shoot regeneration and gradual loss of morphogenic capacity is discussed.

KW - in vitro plant regeneration

KW - maracuya

KW - organogenesis

KW - yellow passion fruit

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U2 - 10.1023/B:TICU.0000049440.10767.29

DO - 10.1023/B:TICU.0000049440.10767.29

M3 - Article

AN - SCOPUS:9644307863

SN - 0167-6857

VL - 79

SP - 87

EP - 90

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 1

ER -

Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa

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Keywords

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